# Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

## TL;DR

This study shows that platelet-rich plasma can reduce replication of certain bovine viruses in cell cultures, suggesting it may be a useful antiviral treatment.

## Contribution

The study demonstrates PRP's virus-, cell-, and dose-dependent modulation of bovine viral replication in vitro.

## Key findings

- 10% PRP reduces extracellular BoAHV-1.1 titers in MDBK and BESc cells.
- PRP shows cell-dependent inhibition of BoGHV-4 and BVDV replication.
- PRP may interfere with viral egress and has broader antiviral effects in BESc cells.

## Abstract

•PRP modulates BoAHV-1.1, BoGHV-4, and BVDV replication in MDBK and BESc cells.•10 % PRP reduces extracellular BoAHV-1.1 titers in both MDBK and BESc cultures.•BoGHV-4 shows cell-dependent responses to PRP, with selective viral inhibition.•PRP emerges as a promising antiviral tool for bovine endometrial viral infections.

PRP modulates BoAHV-1.1, BoGHV-4, and BVDV replication in MDBK and BESc cells.

10 % PRP reduces extracellular BoAHV-1.1 titers in both MDBK and BESc cultures.

BoGHV-4 shows cell-dependent responses to PRP, with selective viral inhibition.

PRP emerges as a promising antiviral tool for bovine endometrial viral infections.

Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle industry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential.

Image, graphical abstract