Mining expansin-like proteins from rumen microbiota and functional characterization of two anaerobic fungal expansin-like proteins

TL;DR

This study explores expansin-like proteins in rumen microbes and shows they help break down plant biomass more efficiently, improving feed sustainability.

Contribution

The study identifies and characterizes ELPs from rumen anaerobic fungi, revealing their role in lignocellulose degradation.

Findings

396 ELPs were identified in the rumen microbiota, showing high diversity, especially in anaerobic fungi.

PFSWO1_1 improved rice straw hydrolysis by 21.6% when combined with cellulases due to its thermal stability and substrate affinity.

ELP pretreatment enhanced corn stover fermentation quality and nutritional value.

Abstract

Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustainability. Expansin-like proteins (ELPs) play essential roles in plant cell wall degradation, yet their functions remain largely underexplored in rumen microbes. The purpose of this study was to investigate the effects of rumen microbial ELPs on lignocellulose degradation.

This study systematically identified 396 ELPs within the rumen microbiota, uncovering remarkable diversity, particularly among anaerobic fungi. Three representative ELPs from Pecoramyces ruminantium F1 (PFLoos_1, PFSWO1_1, PFSWO2_1) were selected for biochemical characterization. While PFSWO2_1 could not be expressed, PFLoos_1 and PFSWO1_1 exhibited significant synergy with cellulases. The CBM10-containing PFSWO1_1 demonstrated superior thermal stability (up to 65 °C) and substrate affinity, increasing rice straw hydrolysis efficiency by 21.6% (reducing sugar yield) compared to cellulase alone. Structural analyses revealed that CBM10 enabled PFSWO1_1 to preferentially bind complex substrates, whereas the single-domain PFLoos_1 targeted simpler substrates. Notably, ELP pretreatment of corn stover significantly improved fermentation quality (pH and lactic acid) and nutritional value (neutral detergent fiber, acid detergent fiber, and water-soluble carbohydrates).

These findings indicate that ELPs are abundant in the rumen and play a synergistic role in lignocellulosic biomass conversion.

The online version contains supplementary material available at 10.1186/s40104-025-01287-6.

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