# Attempts to Establish a Helicobacter bilis Biliary Tract Infection Model via Oral Administration of Helicobacter bilis

## TL;DR

This study attempts to create a biliary tract infection model using Helicobacter bilis in hamsters but finds no evidence of cancer development.

## Contribution

The study provides new insights into the limitations of oral H. bilis administration for inducing biliary tract cancer in an animal model.

## Key findings

- H. bilis was detected in the intestines but not in the biliary tract of hamsters.
- No carcinogenesis was observed in the biliary system of the orally administered group.
- PCR positivity was higher in the H. bilis group but not statistically significant.

## Abstract

Background

Although several studies have reported an association between Helicobacter bilis (H. bilis) and human biliary tract cancer, in vivo studies are necessary to further assess this phenomenon. In this study, we developed an animal model of H. bilis infection, determined whether oral exposure to H. bilis induces biliary carcinogenesis, and evaluated colonization and pathological changes.

Methods

Seven-week-old female Syrian golden hamsters were administered H. bilis via the intragastric route using a sonde and euthanized after two, four, 12, 24, and 48 weeks. We assessed the rate of bacterial colonization in the intrahepatic bile ducts and gallbladders, polymerase chain reaction (PCR) positivity rate using H. bilis-specific primers, carcinogenic or pathological alterations, and cholangitis scores.

Results

H. bilis was detected in the intestinal tract of hamsters but not in the biliary tract. PCR positivity was higher in the H. bilis group than in the control group at each time point, but not statistically significant. Histological alterations in the biliary system of the orally administered group were limited to gallbladder hyperplasia, with no evidence of carcinogenesis. The cholangitis scores were similar between the two groups.

Conclusions

In this study, the oral administration of H. bilis alone did not induce carcinogenesis. Because human epidemiological studies have revealed that H. bilis is involved in carcinogenesis, further research is needed to elucidate the relationship between H. bilis and human biliary tract cancer.