Anatomical variations along the leaf axis modulate photosynthetic responses of sorghum and maize under different water availabilities

TL;DR

This study compares how drought affects leaf anatomy and photosynthesis in sorghum and maize, finding that sorghum adapts better to water stress than maize.

Contribution

The study reveals how anatomical and photosynthetic traits along the leaf axis differ between sorghum and maize under drought conditions.

Findings

Sorghum increased leaf thickness and photosynthetic efficiency under drought, while maize showed reductions.

The middle region of leaves was most responsive to drought in both species.

Maize had higher stomatal density and transpiration rates compared to sorghum under water limitation.

Abstract

Water limitation leads to alterations in plants, including tolerance responses. Maize and sorghum are both C4 crops with contrasting drought tolerance, where several aspects of leaf anatomy and physiology are unclear. This work aimed to investigate the effect of drought on anatomical and photosynthetic traits along the leaf axis of maize and sorghum.Experiments were conducted in a greenhouse with maize and sorghum exposed to three irrigation conditions (field capacity (FC), 75% FC, and 50% FC). Three leaf regions (base, middle, and tip) were assessed for photosynthetic and anatomical parameters.Water limitation promoted reductions in maize leaves in terms of water‐use efficiency, leaf thickness, xylem vessel diameter, and area of the bundle sheath; however, sorghum leaves increased these under the same conditions. The middle region of the leaf had higher values than other leaf parts for most parameters, while sorghum had increased ΦPSII, Fv/Fm, and ETR at the leaf base. Photochemical values increased in both species under water limitation. Maize had increased stomatal density compared to sorghum, which led to higher transpiration rates.Anatomical and photosynthetic traits varied along the leaf axis and were more reduced in maize than sorghum under drought. The middle region of the leaf was most responsive to these changes in both species.

Water limitation leads to alterations in plants, including tolerance responses. Maize and sorghum are both C4 crops with contrasting drought tolerance, where several aspects of leaf anatomy and physiology are unclear. This work aimed to investigate the effect of drought on anatomical and photosynthetic traits along the leaf axis of maize and sorghum.

Experiments were conducted in a greenhouse with maize and sorghum exposed to three irrigation conditions (field capacity (FC), 75% FC, and 50% FC). Three leaf regions (base, middle, and tip) were assessed for photosynthetic and anatomical parameters.

Water limitation promoted reductions in maize leaves in terms of water‐use efficiency, leaf thickness, xylem vessel diameter, and area of the bundle sheath; however, sorghum leaves increased these under the same conditions. The middle region of the leaf had higher values than other leaf parts for most parameters, while sorghum had increased ΦPSII, Fv/Fm, and ETR at the leaf base. Photochemical values increased in both species under water limitation. Maize had increased stomatal density compared to sorghum, which led to higher transpiration rates.

Anatomical and photosynthetic traits varied along the leaf axis and were more reduced in maize than sorghum under drought. The middle region of the leaf was most responsive to these changes in both species.

The middle part of C4 leaves is more anatomically and photochemically drought‐adapted than the apex or base. Water limitation did not negatively effect leaf anatomy and photosynthesis in sorghum, but reduced gas exchange and the thickness of leaf tissues in maize.

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