# Comparative Evaluation of the Shear Bond Strength of Self-Adhesive and Glass Ionomer Cement to Dentin After Removal of Hemostatic Agents Using Different Cleansing Protocols: An In Vitro Study

**Authors:** Hemashree Namburajan, Mathew Chalakuzhiyil Abraham, Vidhyasankari N, Rajkumar K, Abhinayaa Suthagar, Vishnupriya Venkatasubramanian, Sindhuja Nagarajan

PMC · DOI: 10.7759/cureus.95030 · 2025-10-21

## TL;DR

This study compares how well different cleaning methods remove hemostatic agents from dentin and affect the bond strength of two dental cements.

## Contribution

The study introduces a comparative evaluation of EDTA and dentin conditioner for removing hemostatic agents and their impact on bond strength of SARC and GIC.

## Key findings

- EDTA provided the highest bond strength for SARC after both FeSO4 and AlCl3 contamination.
- Dentin conditioner was most effective for GIC after AlCl3 contamination.
- Control groups had consistently lower bond strength values.

## Abstract

Background

Hemostatic agents (HA), such as aluminium chloride (AlCl3) and ferric sulfate (FeSO4), can affect the dentin by obstructing adhesive penetration, and reducing the bond strength of self-adhesive resin cements (SARC) and glass ionomer cements (GIC).

Aim

To evaluate and compare the shear bond strength (SBS) of bonding and luting agents (SARC and GIC) to dentin after the removal of AlCl3 and FeSO4, using different cleansing protocols (dentin conditioner (DC) and ethylenediaminetetraacetic acid (EDTA)), and to determine whether the same cleansing protocol can be applied for both bonding and luting procedures.

Methodology

Sixty molar teeth were sectioned and categorized into two HA groups and three cleansing-protocol subgroups. Within each subgroup, specimens were bonded with SARC and GIC. SBS was evaluated, and data were statistically analyzed using independent t-tests, one-way and three-way analysis of variance (ANOVA), with the level of significance established at p < 0.05

Results

For SARC, EDTA yielded the highest SBS after both FeSO4 and AlCl3 contamination, followed by DC and control (p < 0.001). For GIC, DC produced the greatest SBS after AlCl3 contamination (p = 0.042), whereas EDTA was most effective after FeSO4 contamination (p = 0.017). Control groups consistently showed the lowest SBS values.

Conclusion

EDTA is the preferred cleanser for SARC after use of both contaminants, and for GIC following FeSO4 contamination, while DC was preferred for GIC after AlCl3.

## Linked entities

- **Chemicals:** AlCl3 (PubChem CID 24012), FeSO4 (PubChem CID 24393), EDTA (PubChem CID 6049)

## Full-text entities

- **Chemicals:** DC (-), AlCl3 (MESH:D000077410), EDTA (MESH:D004492), ferric sulfate (MESH:C024823), Glass Ionomer (MESH:C015897)

## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12631152/full.md

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Source: https://tomesphere.com/paper/PMC12631152