# ERGA-BGE reference genome of Hanak's bat ( Pipistrellus hanaki), an IUCN Vulnerable species restricted to forest-like biotopes

**Authors:** Panagiotis Georgakakis, Danae Karakasi, Petros Lymberakis, Manolis Papadimitrakis, Manos Stratakis, Eleftherios Bitzilekis, Nikolaos Poulakakis, Astrid Böhne, Rita Monteiro, Rosa Fernández, Nuria Escudero, Manon Angel, Manon Angel, Jean-Marc Barbance, Julie Batisse, Odette Beluche, Laurie Bertrand, Elodie Brun, Maria Dubois, Corinne Dumont, Barbara Estrada, Thomas Guerin, Zineb El Hajji, Sandrine Lebled, Patricia Lenoble, Claudine Louesse, Ghislaine Magdelenat, Eric Mahieu, Claire Milani, Sophie Oztas, Marine Paillard, Emilie Payen, Emanuelle Petit, Murielle Ronsin, Benoit Vacherie, Alice Moussy, Corinne Cruaud, Karine Labadie, Lola Demirdjian, Sophie Mangenot, Caroline Belser, Patrick Wincker, Pedro H. Oliveira, Jean-Marc Aury, Leanne Haggerty, Swati Sinha, Fergal Martin, Chiara Bortoluzzi, Richard Orton

PMC · DOI: 10.12688/openreseurope.20937.1 · 2025-09-25

## TL;DR

This paper presents the reference genome of Hanak's bat, a vulnerable species found only in Libya and Greece, to help understand its genetic makeup and conservation needs.

## Contribution

The study provides the first chromosome-level reference genome for Hanak's bat.

## Key findings

- The genome assembly includes 23 chromosomal pseudomolecules and spans 1.9 Gb.
- Contig and scaffold N50 values are 48.7 Mb and 89.1 Mb, respectively, indicating high quality.
- The genome will aid in studying the species' phylogenetic history and genetic isolation.

## Abstract

Hanak's bat (
Pipistrellus hanaki Hulva and Benda 2004) is one of the most range restricted mammals in Europe, since it occurs only in Cyrenaica, Libya, and Crete (Greece). It is currently classified as 'Vulnerable' on the IUCN Red List, with its foraging habitat threatened by a number of human activities. The reference genome of Hanak's bat (
Pipistrellus hanaki) will provide a crucial resource for uncovering the species phylogenetic history and will help assess the degree of genetic isolation among its populations. A total of 23 contiguous chromosomal pseudomolecules (sex chromosomes included) were assembled from the genome sequence. This chromosome-level assembly encompasses 1.9 Gb, composed of 447 contigs and 141 scaffolds, with contig and scaffold N50 values of 48.7 Mb and 89.1 Mb, respectively.

## Linked entities

- **Species:** Pipistrellus hanaki (taxon 412090)

## Full-text entities

- **Species:** Homo sapiens (human, species) [taxon 9606], Pipistrellus hanaki (Hanaki's dwarf bat, species) [taxon 412090]

## Figures

2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12631070/full.md

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Source: https://tomesphere.com/paper/PMC12631070