# Proof of concept for the usability of the IRRAMICE for proton preclinical radiobiological research by a traceable dosimetry evaluation

## TL;DR

This study shows that the IRRAMICE system can reliably deliver proton doses to small targets for preclinical radiobiology experiments.

## Contribution

The IRRAMICE system is demonstrated as a usable and traceable solution for proton-based preclinical animal irradiation.

## Key findings

- Alanine pellet measurements showed doses within 2% of the planned dose.
- Film-based gamma analysis showed 98.3% passing rate for dose distribution accuracy.
- IRRAMICE enables reliable and reproducible treatment plan delivery for preclinical experiments.

## Abstract

Radiobiology relies on animal studies to demonstrate efficacy and safety of treatments. For proton-based studies in clinical settings, bespoke solutions, enabling animal irradiations without compromising health and safety regulations, need to be developed to facilitate the research. This study aims to demonstrate the usability of the IRRAMICE, a novel system with bespoke collimators to effectively treat small targets, within proton clinical facilities. Following a clinical workflow of planning CT, treatment plan design and delivery, a dosimetric evaluation of the IRRAMICE was conducted. Traceable absorbed dose was determined by measurements with (i) ion chamber, (ii) alanine pellets within a mouse phantom and a bespoke holder in the IRRAMICE, and (iii) Gafchromic film in the holder for a relative evaluation of the collimation system. Dose determined with alanine pellets in the mouse phantom were within 2% of the planned dose. The 2%/2 mm local gamma analysis of the dose distribution of the collimated beam spot determined by the film and the treatment planning system showed an average 98.3% passing rate. Through measurements in mouse phantoms, IRRAMICE demonstrated to be a suitable device for enabling the setup and delivery of treatment plans in a reliable and reproducible manner, to facilitate in vivo preclinical experiments.

The online version contains supplementary material available at 10.1038/s41598-025-24546-y.