# Amorphous carbonized objects and their contribution to reconstructing ancient Mesoamerican cuisine: An innovative non-destructive methodological approach

**Authors:** Clarissa Cagnato, Nawa Sugiyama, Laura Longo, Alessandro Bonetto, Matteo Parisatto, Elena Longo, Marko Prasek, Giuliana Tromba, Antonio Marcomini, Elena Badetti

PMC · DOI: 10.1371/journal.pone.0334457 · 2025-11-19

## TL;DR

This paper introduces a non-destructive method using imaging techniques to study ancient food remains in Mesoamerica, helping reconstruct ancient recipes and culinary traditions.

## Contribution

The study presents a novel pipeline combining multiple imaging techniques to analyze amorphous carbonized objects in Prehispanic Central American archaeological contexts.

## Key findings

- The pipeline successfully identified complex food remains from the ancient site of Teotihuacan.
- This is the first use of combined imaging techniques to distinguish food remains from plant parts in Mesoamerican archaeology.
- The method can be applied more broadly to study ancient culinary traditions in Mesoamerica.

## Abstract

Archaeobotanists often come across small, amorphous carbonized objects (ACOs) in their flotation samples. Although their identification remains difficult and requires a range of characterization techniques, the study of ACOs recovered from sites in Europe and the Levant have allowed researchers to reconstruct ancient recipes. However, similar materials from sites in pre-Hispanic Central America have been overlooked, hampering our understanding of their ancient cooking traditions. This article proposes a new pipeline to study such remains through three types of non-destructive imaging techniques: optical microscopy (OM), scanning electron microscopy (SEM), and synchrotron radiation-based phase-contrast X-ray computed microtomography (SR micro-CT), key techniques to consider for the imaging of archaeological materials. The approach was developed by establishing a reference collection from modern foods based on traditional ingredients (e.g., maize, manioc) used in the region of interest. This pipeline was then tested on archaeological samples from the ancient site of Teotihuacan (Mexico), which successfully captured the presence of the remains of complex, multi-component food preparations from a feasting context. To the best of our knowledge, this is the first time in which the combination of imaging techniques has been used to discriminate between actual food remains, or simply seeds or plant parts in archaeological contexts from Prehispanic Central America. This study allows to shed more light on ancient recipes and culinary traditions and can be applied more broadly to other contexts in Mesoamerica.

## Full-text entities

- **Diseases:** OM (MESH:D009901), fire (MESH:D000092422), swelling (MESH:D004487), ACOs (MESH:C567546)
- **Chemicals:** aluminium (MESH:D000535), silicon (MESH:D012825), ACO (-), fat (MESH:D005223), sugars (MESH:D000073893), charcoal (MESH:D002606), methane (MESH:D008697), starch (MESH:D013213), water (MESH:D014867), lipids (MESH:D008055), calcium oxalate (MESH:D002129)
- **Species:** Bolboschoenus glaucus (species) [taxon 1815958], Cicer arietinum (chickpea, species) [taxon 3827], Phaseolus vulgaris (common bean, species) [taxon 3885], Manihot esculenta (cassava, species) [taxon 3983], Bolboschoenus maritimus (species) [taxon 76417], Zea mays (maize, species) [taxon 4577], Homo sapiens (human, species) [taxon 9606], Theobroma cacao (cacao, species) [taxon 3641], Opuntia (prickly-pears, genus) [taxon 106975], Physalis ixocarpa (tomatillo, species) [taxon 374031], Amaranthus caudatus (amaranth, species) [taxon 3567], Lathyrus oleraceus (garden pea, species) [taxon 3888], Chenopodium (genus) [taxon 3558], Hordeum sp. (species) [taxon 50472], Salvia hispanica (species) [taxon 49212], Persea americana (avocado, species) [taxon 3435]

## Figures

13 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12629468/full.md

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Source: https://tomesphere.com/paper/PMC12629468