Antiaging, Wound‐Healing Properties and Chemical Characterization of Crude Hydroalcoholic Extract and Fractions of Myrcia neoobscura

TL;DR

This study explores the antiaging and wound-healing properties of extracts from Myrcia neoobscura leaves, highlighting their rich phenolic content and potential for phytocosmetic use.

Contribution

The study reports the first identification of several phenolic compounds in the Myrcia genus and evaluates their antiaging and wound-healing potential.

Findings

CHE and AF showed the best antioxidant and collagenase/elastase inhibition results.

EAF exhibited high oxidative glycation inhibition comparable to quercetin.

CHE promoted significant fibroblast migration and was non-irritating in skin tests.

Abstract

The aim of this study was to characterize the phytochemical profile and potential antiaging and wound‐healing activities of 70% hydroalcoholic crude extract (CHE) from Myrcia neoobscura leaves and its fractions—insoluble (IF), ethyl acetate (EAF), and aqueous (AF)—for use in phytocosmetics for skin application. CHE and its fractions showed high concentrations of total phenolics, including flavanols and flavonoids. Ten phenolic compounds were identified, with gallic acid as the major, followed by chlorogenic and p‐coumaric acids. Catechin, isoorientin, taxifolin, resveratrol, naringenin, and eriodictyol were reported for the first time in the genus. CHE and AF showed the best results in antioxidant assays. In antiglycation activity, EAF showed the highest oxidative glycation inhibition, comparable to quercetin. CHE and AF exhibited the strongest collagenase and elastase inhibition. CHE, IF, and EAF showed the highest SPF. CHE promoted 81.86% fibroblast migration in 24 h, indicating strong wound‐healing potential. CHE maintained cellular viability in the MTT assay. In the HET‐CAM assay and agarose overlay assays, CHE and fractions were classified as non to mildly irritating. These results suggest potential antiaging applications, mainly attributed to the phenolic profile.

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