# Extraction of Triterpene Compounds From Hancornia speciosa Gomes Fruits and Evaluation of Pharmacological Potential

## TL;DR

This study explores the extraction of beneficial triterpene compounds from Hancornia speciosa fruits and evaluates their anti-inflammatory potential.

## Contribution

The study introduces the EDGE extraction method as a more efficient and sustainable alternative for obtaining triterpene-rich extracts.

## Key findings

- EDGE extraction reduced solvent volume by 60 mL and extraction time by 87 minutes compared to ultrasound methods.
- The extract showed no cytotoxicity and significantly reduced reactive oxygen species in neutrophils.
- Lupeol concentration in the extract was comparable between EDGE and ultrasound methods.

## Abstract

Hancornia speciosa Gomes, a Brazilian native species, holds biotechnological potential due to compounds such as lupeol, α‐amyrin, and β‐amyrin, which possess pharmacological properties. This study investigates triterpene‐rich extracts from H. speciosa fruit for potential anti‐inflammatory applications. Extraction was performed using the energized‐guided dispersive extraction (EDGE) method and compared to ultrasound extraction techniques to evaluate efficiency and yield. The EDGE system demonstrated notable advantages over ultrasound extraction, including a 60 mL reduction in solvent volume and an 87‐min decrease in extraction time, enhancing efficiency, cost‐effectiveness, and sustainability. The EDGE extraction successfully obtained a triterpene‐rich extract from H. speciosa Gomes with a concentration of lupeol (50.09 ± 1.29 mg/g) with no significant difference when compared to the extract obtained by ultrasound (49.95 ± 2.54 mg/g). The extract showed no cytotoxicity and irritation in the hen's egg test‐chorioallantoic membrane assay and significantly reduced reactive oxygen species produced by neutrophils. These results indicate the extract's phytotherapeutic potential, suggesting new possibilities for future studies and therapeutic applications, and highlight EDGE as a promising technique for phytotherapeutic applications.