# Unveiling the Chemical Profile of Teucrium chamaedrys subsp. gracile (Batt.) Rech.F. dried Aerial Parts From Algeria

## TL;DR

This study presents the first chemical analysis of Teucrium chamaedrys subsp. gracile from Algeria, identifying key compounds in its aerial parts.

## Contribution

The paper provides the first detailed phytochemical profile of Teucrium chamaedrys subsp. gracile using multiple analytical techniques.

## Key findings

- Palmitic acid was the major fatty acid identified in the sample.
- β-caryophyllene and humulene were the dominant volatile compounds.
- Five non-volatile compounds, including teucrioside and chlorogenic acid, were characterized.

## Abstract

In this paper, the first phytochemical analysis on Teucrium chamaedrys subsp. gracile was reported. The dried aerial parts collected in Algeria were studied, and the analysis was conducted using different techniques: gas chromatography/mass spectrometry (GC/MS) for the fatty acid content, headspace solid‐phase microextraction‐GC‐MS for the volatile composition, column chromatography along with nuclear magnetic resonance spectroscopy, and MS for non‐volatile pattern characterization. Saturated fatty acids were found to be prevalent, with palmitic acid as the major compound. In the volatile fraction, β‐caryophyllene and humulene, comprised in the class of sesquiterpenes, were the major components. Indeed, five non‐volatile compounds were observed, namely teucrioside (1), cirsiliol (2), harpagide (3), and chlorogenic acid (4). The employed analytical techniques have proven to be suitable for producing reliable and reproducible results in the description of the phytochemical profile of the matrix.