Antinociceptive and Anti‐inflammatory Effect of Nicandrin B Isolated From Datura ferox in Zebrafish
Jéssica Bezerra Maciel, Hortência Ribeiro Liberato, Antônio Wlisses da Silva, Maria Auxiliadora Solange Silva Gondim Pereira, Emanuela de Lima Rebouças, Francisco das Chagas L. Pinto, Matheus Nunes da Rocha, Maria Kueirislene Amâncio Ferreira
TL;DR
Nicandrin B, a compound from Datura ferox, shows pain-relieving and anti-inflammatory effects in zebrafish without toxicity.
Contribution
Nicandrin B's antinociceptive and anti-inflammatory mechanisms via TRPA1 and TRPV1 are demonstrated in a zebrafish model.
Findings
Nicandrin B reduced nociception in zebrafish and was reversed by TRPA1 and TRPV1 antagonists.
It attenuated inflammation by reducing edema, neutrophil recruitment, and reactive oxygen species.
Docking analysis confirmed strong binding affinity of Nic B with TRPA1.
Abstract
Nicandrin B (Nic B), a withanolide isolated from Datura ferox leaves, was investigated for its antinociceptive and anti‐inflammatory effects in adult zebrafish (Danio rerio). Animals treated with Nic B (4, 20, and 40 mg/kg) showed no toxicity and maintained normal locomotor activity. The compound significantly reduced nociception induced by formalin and hypertonic saline; these effects were reversed by TRPA1 and transient receptor potential vanilloid 1 antagonists, indicating neuromodulation of these targets. Additionally, Nic B attenuated carrageenan‐induced abdominal edema, reduced neutrophil recruitment, and decreased hepatic reactive oxygen species levels. Docking analysis confirmed a favorable binding affinity with TRPA1, supporting its therapeutic potential. These findings suggest that Nic B combines analgesic, anti‐inflammatory activity, and toxicological safety, making it a…
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Taxonomy
TopicsPhytochemicals and Medicinal Plants · Ion Channels and Receptors · Calpain Protease Function and Regulation