Dentin matrix protein-1 promoted osteogenic differentiation of valvular interstitial cells via MAPK signal pathway during aortic valve calcification
Jinjie Chen, Yefan Jiang, Si Chen, Junxiang Liu, Wenjing Zhang, Yixuan Wang, Geng Li
TL;DR
This study shows that Dentin matrix protein-1 promotes bone-like changes in heart valve cells through a specific signaling pathway, contributing to aortic valve disease.
Contribution
The novel finding is that DMP-1 promotes osteogenic differentiation of VICs via the integrin αvβ3 and MAPK pathway during aortic valve calcification.
Findings
DMP-1 expression is significantly increased in calcified aortic valves.
DMP-1 induces osteogenic differentiation in VICs through integrin αvβ3 and activates the MAPK pathway.
RGD peptides of DMP-1 are involved in promoting osteogenic gene expression in VICs.
Abstract
Valvular interstitial cells (VICs) are integral to the progression of calcific aortic valve disease (CAVD). Dentin matrix protein-1 (DMP-1), a member of the Sibling family protein, is implicated in the calcification process. This study aims to investigate the role and mechanisms of DMP-1 in the osteogenic differentiation of VICs. Between April 2018 and December 2018, aortic valve tissues were collected from 14 patients undergoing aortic valve replacement or heart transplantation. DMP-1 expression was quantified in calcified valves versus normal controls. An in vitro model of VICs’ osteogenic differentiation was established to study the regulatory mechanism of DMP-1 on valvular calcification using immunoblot, immunohistochemistry, immunofluorescence, etc. The expression of DMP-1 was significantly increased in the calcified aortic valves patients (P < 0.01). DMP-1, both short and long…
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Taxonomy
TopicsCardiac Valve Diseases and Treatments · Infective Endocarditis Diagnosis and Management · Oral microbiology and periodontitis research