Cell-Derived Nanocarriers of Apoptotic Bodies with an Antimicrobial Peptide for Targeting Intracellular S. aureus Infections
Valentina Nieto-Marín, Ian Alejandro Fernandez-Soliz, Jorge William Arboleda Valencia, Daniel Pletzer, Danieli Fernanda Buccini, Octávio Luiz Franco
TL;DR
Researchers developed a new nanocarrier to deliver an antimicrobial peptide to treat hard-to-reach Staphylococcus aureus infections inside cells.
Contribution
This is the first study to develop a ReApoBD-AMP formulation for targeting intracellular S. aureus infections.
Findings
ReApoBDs achieved high encapsulation efficiency (∼70%) and improved intracellular delivery of BotrAMP14.
The nanoformulation reduced intracellular bacterial loads with lower cytotoxicity than free peptide.
In vivo experiments showed ReApoBD-BotrAMP14 reduced dermonecrosis and SCV prevalence more effectively than conventional treatments.
Abstract
Intracellular infections caused by Staphylococcus aureus are challenging due to their ability to evade host defenses, through the development of small colony variants (SCVs) that are resistant to conventional therapies. This study yielded and evaluated size-remodeled apoptotic bodies (ReApoBDs) as cell-derived nanocarriers for the targeted delivery of BotrAMP14, an antimicrobial peptide, to treat S. aureus-infected macrophages. ReApoBDs demonstrated high encapsulation efficiencies (∼70%), biocompatibility, sustained drug release over 12 h, colloidal stability, and improved intracellular delivery. The ReApoBDs-BotrAMP14 nanoformulation reduced intracellular bacterial loads while exhibiting lower cytotoxicity compared to the free peptide. Moreover, in vivo experiments demonstrated that ReApoBD-BotrAMP14 reduced dermonecrosis by 31.7% and SCV prevalence by 72.9%, more effectively than…
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Taxonomy
TopicsNanoplatforms for cancer theranostics · Antimicrobial Peptides and Activities · RNA Interference and Gene Delivery