High-throughput developmental assay of cold tolerance in Caenorhabditis elegans
Amanda L. Peake, Nikita S. Jhaveri, Erik C. Andersen, John R. Stinchcombe
TL;DR
The paper introduces a high-throughput method to study cold tolerance in C. elegans larvae, revealing genetic variation and heritability.
Contribution
A scalable high-throughput assay for measuring cold tolerance in C. elegans during the L1 larval stage.
Findings
Animals exposed to 24-hour cold treatment showed greater variation in cold tolerance.
The assay is suitable for large-scale genetic and evolutionary studies of cold tolerance in nematodes.
Abstract
Temperature can impose strong selection causing thermal tolerance variation between individuals, populations, and species. We developed a high-throughput larval development assay for cold tolerance in the model organism Caenorhabditis elegans . We exposed animals to 4°C cold treatments for either 12 or 24 hours. Animals exposed to the 24-hour cold treatment exhibited greater variation and heritability in cold tolerance during the L1 larval stage. The high-throughput approach that we developed is easily scalable to simultaneously measure a large number of strains, which makes it ideal for studying the genetics and evolution of cold tolerance in Caenorhabditis nematodes.
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Taxonomy
TopicsGenetics, Aging, and Longevity in Model Organisms · Physiological and biochemical adaptations · Evolution and Genetic Dynamics