# Brain metabolic alterations in individuals with chronic non-specific neck pain assessed using proton magnetic resonance spectroscopy

## TL;DR

This study uses brain scans to find metabolic changes in people with chronic neck pain, linking these changes to pain severity and duration.

## Contribution

The study identifies specific brain metabolite alterations in chronic non-specific neck pain using proton magnetic resonance spectroscopy.

## Key findings

- Decreased myo-inositol and N-acetylaspartate levels were found in pain-related brain regions of individuals with neck pain.
- Altered metabolite levels correlated with pain intensity, duration, and disability in chronic neck pain patients.
- Increased choline levels in the somatosensory cortex were observed in individuals with chronic neck pain.

## Abstract

Altered brain metabolites in pain-related regions provide insights into the underlying mechanisms of chronic pain. However, brain metabolites alterations in chronic non-specific neck pain remain unknown. This study aimed to investigate brain metabolite concentrations in individuals with chronic non-specific neck pain and their relationships with pain-related outcomes. Participants included 30 individuals with chronic non-specific neck pain and 30 pain-free controls. Absolute concentrations and metabolite ratios of total creatine (tCr), choline (Cho), myo-inositol (mI), N-acetylaspartate (NAA) and glutamate/glutamine (Glx) were measured in regions involved in pain processing and modulation, including dorsolateral prefrontal cortex (DLPFC), primary somatosensory cortex (S1), insula and thalamus, using proton magnetic resonance spectroscopy (1H-MRS). Compared to controls, participants with neck pain exhibited decreased mI and mI/tCr (left DLPFC and thalamus), NAA and NAA/tCr (right S1) and Glx and Glx/tCr (right DLPFC) and increased Cho and Cho/tCr (left S1) (adjusted p-values < 0.05). Altered metabolite levels were correlated with pain duration, intensity, extent, disability and PPT at C2-3 (r ranged from − 0.48 to 0.55, adjusted p-values < 0.05). The results suggest that ¹H-MRS detects altered levels of mI, NAA, Glx and Cho in specific brain regions involved in pain regulation, which may contribute to the persistence of neck pain.