# PPI-based screening of hub genes related to sepsis migration/pyroptosis and immune infiltration analysis

## TL;DR

This study identifies key genes and miRNA interactions linked to sepsis and immune responses, offering potential biomarkers and therapeutic targets.

## Contribution

Novel identification of sepsis-related hub genes and miRNA interactions through PPI and immune infiltration analysis.

## Key findings

- 3566 differentially expressed genes were identified, with 23 linked to pyroptosis/migration.
- Eight key genes (e.g., TLR2, SIRT1) remained after validation, showing potential as sepsis biomarkers.
- Immune infiltration analysis revealed varied effects on MAPK14 and roles of miRNA dysregulation in sepsis.

## Abstract

Sepsis incidence is rising, but its pathogenesis remains unclear. This study aimed to identify therapeutic targets.

Three GEO datasets (GSE154918, GSE32707, GSE54514) were analyzed after batch correction. Differentially expressed genes (DEGs) were identified, and those related to pyroptosis/migration were cross-analyzed. Functional enrichment (GO/KEGG) and interaction networks (PPI, mRNA-miRNA, mRNA-TF, mRNA-drug) were constructed. External validation used GSE57065, excluding non-significant genes. ROC analysis and immune infiltration were performed.

3566 DEGs (1715 up, 1851 down) were identified, with 23 linked to pyroptosis/migration. Enrichment analysis highlighted roles in cell adhesion, cytokine regulation, and pathways like IL-17, TNF, and Toll-like receptor signaling. mRNA-miRNA interactions for 12 key genes were predicted. After validation, eight key genes remained: TLR2, SIRT1, PTGS2, MAPK14, IL18, ICAM1, CD274, and CASP3. Immune infiltration revealed varied effects on MAPK14.

Key gene alterations may serve as sepsis biomarkers, and miRNA dysregulation could play a critical role in sepsis pathophysiology.