Trans‐Synaptic Virus Tracing Reveals Reciprocal Connections of the Interpeduncular Nucleus With the Mood, Exteroceptive, Interoceptive, and Motor Control Systems
Audrey K. Wong, Bor‐Wei Cherng, Hisaya Kakinuma, Claire Wyart, Hitoshi Okamoto
TL;DR
This study maps the brain connections of the interpeduncular nucleus in zebrafish, showing how it integrates sensory and emotional information for decision-making and stress resilience.
Contribution
The study reveals reciprocal connections of the IPN with sensory, emotional, and motor systems using advanced viral tracing techniques.
Findings
The IPN connects with interoceptive, exteroceptive, and motor systems in zebrafish.
Emotional regulatory systems like serotonin and dopamine neurons are linked to the IPN.
The Hb–IPN–GC circuit integrates multimodal information for behavioral responses.
Abstract
The interpeduncular nucleus (IPN) is an evolutionarily conserved brain nucleus that receives direct input from the habenula (Hb). In zebrafish (Danio rerio), it is involved in decision‐making dependent on the idiothetic perception of the status of the body, such as head direction and posture. It is also known that the potentiation of the Hb–IPN–griseum centrale (GC) circuit makes fish resilient to stress in coping with fear and social conflict. To address why the same neural circuit controls these two distinctive physiological aspects, we performed anterograde and retrograde trans‐synaptic viral tracing, retrograde mono‐synaptic viral tracing, and lipophilic dye tracing to map the connectivity from the dorsal and intermediate IPN. We revealed reciprocal connections of d/iIPN and GC neurons with multiple brain regions for sensory inputs, including interoceptive systems that receive…
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Taxonomy
TopicsZebrafish Biomedical Research Applications · Marine animal studies overview · Marine Invertebrate Physiology and Ecology