# Genome sequence of Mycobacterium smegmatis phage Mao1

## TL;DR

This paper reports the genome sequence of a new bacteriophage called Mao1, which is closely related to another phage called Sejanus.

## Contribution

The study provides a detailed genome analysis of the newly isolated temperate bacteriophage Mao1.

## Key findings

- Mao1 has a genome of 65,240 base pairs with 101 confirmed genes and a high GC content of 66.3%.
- Mao1 shares 99.79% nucleotide identity with phage Sejanus and belongs to cluster AD.
- The genome lacks tRNA genes, a notable feature for further study.

## Abstract

Mao1, a temperate bacteriophage isolated in North Georgia, was found to have a 65,240 base pair genome with 101 confirmed genes, no tRNA genes, and a 66.3% guanine–cytosine content. It shares 99.79% nucleotide identity with phage Sejanus. Bacteriophages that share over 50% identities are grouped into clusters, with Mao1 being in cluster AD.