MRanalysis: a comprehensive online platform for integrated, multimethod Mendelian randomization and associated post-GWAS analyses

TL;DR

MRanalysis is a web-based platform that simplifies Mendelian randomization analysis using GWAS data, making it accessible to non-experts.

Contribution

MRanalysis and GWASkit provide a user-friendly, no-code solution for integrated MR analysis and GWAS data preprocessing.

Findings

MRanalysis supports univariable, multivariable, and mediation MR analyses through an intuitive interface.

GWASkit offers higher accuracy and efficiency in GWAS data preprocessing compared to existing tools.

Case studies demonstrate the real-world utility and efficiency of MRanalysis and GWASkit.

Abstract

Mendelian randomization (MR) is a powerful epidemiological method for inferring causal relationships between exposures and outcomes using genome-wide association study (GWAS) data. However, its adoption is limited by inconsistent data formats, lack of standardized workflows, and the need for programming expertise. To address these challenges, we developed MRanalysis, a user-friendly, web-based platform for integrated MR analysis, and GWASkit, a standalone tool for GWAS data preprocessing.

MRanalysis provides a comprehensive, no-code workflow for MR analysis, including data quality assessment, power estimation, single-nucleotide polymorphism to gene enrichment, and visualization. It supports univariable, multivariable, and mediation MR analyses through an intuitive interface. GWASkit facilitates rapid GWAS data preprocessing, such as rs ID conversion and format standardization, with significantly higher accuracy and efficiency than existing tools. Case studies demonstrate the utility and efficiency of both tools in real-world scenarios.

MRanalysis and GWASkit lower barriers to MR analysis, making it more accessible, reliable, and efficient. By democratizing MR, these tools can accelerate discoveries in genetic epidemiology, inform public health strategies, and guide targeted interventions. MRanalysis is freely available at https://mranalysis.cn, and GWASkit can be accessed at https://github.com/Li-OmicsLab-MPU/GWASkit. Together, they represent a significant advance in understanding the complex relationships between genes, exposures, and health outcomes.

Graphical Abstract

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