Metabolomic Profiling of Citrus Grafts Challenged by Phytophthora citrophthora: Using the Same Samples Previously Analyzed for Epigenetic Responses
Felipe Hilario, Luciano da Silva Pinto, Adielle Rodrigues da Silva, João Batista Fernandes, Abelmon da Silva Gesteira, Maria Fátima das Graças Fernandes da Silva
TL;DR
This study uses metabolomics to analyze how citrus grafts respond to a pathogen, revealing chemical changes that could help in early disease detection.
Contribution
The novel contribution is a metabolomic analysis of citrus grafts infected with Phytophthora citrophthora, using the same samples previously studied for epigenetic responses.
Findings
Infection induced metabolic shifts, including increased levels of coumarins, flavonoids, and alkaloids.
Sixty-six metabolites were annotated, with their distribution varying by graft combinations and inoculation regimes.
Chemical profiles did not directly correlate with prior DNA methylation patterns but support metabolomics for early gummosis detection.
Abstract
Phytophthora citrophthora infection severely affects citrus crops, demanding improved diagnostic and control strategies. In a previous study, epigenetic responses, including DNA methylation patterns, were analyzed in grafted citrus plants exposed to this pathogen. Here, we report a complementary metabolomic analysis using the same biological material, stored under cryogenic conditions to preserve chemical integrity. “Pera” sweet orange and “Tahiti” acid lime scions grafted onto contrasting rootstocks were inoculated and reinoculated with P. citrophthora. Untargeted metabolomics via LC-MS/MS and molecular networking revealed infection-induced metabolic shifts, including increased levels of coumarins, flavonoids, and alkaloids potentially involved in plant defense. Sixty-six metabolites were annotated, and their distribution varied according to graft combinations and inoculation regimes.…
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Taxonomy
TopicsPlant Pathogens and Resistance · Plant Disease Management Techniques · Plant tissue culture and regeneration