# Assessment of bacterial proteome remodeling under virulence-inducing conditions for molecular pharming applications

## TL;DR

The study explores how bacteria change their proteins when triggered by virulence-inducing conditions, which could help in making plant-based pharmaceuticals.

## Contribution

Leaf extracts are proposed as a cost-effective alternative to acetosyringone for inducing virulence in bacteria for molecular pharming.

## Key findings

- Proteomics revealed increased abundance of virulence proteins in Agrobacterium tumefaciens after exposure to inducers.
- Leaf extract from Nicotiana benthamiana is a potential low-cost alternative to acetosyringone for virulence induction.

## Abstract

Using mass spectrometry-based proteomics, we identified virulence proteins from Agrobacterium tumefaciens with significantly increased abundance following exposure to chemical (acetosyringone) and biological (leaf extract from Nicotiana benthamiana) inducers. Leaf extracts could be a cost-effective alternative to acetosyringone for inducing pathogen virulence for the molecular pharming industry.