# Characterization of four cluster A1 Mycobacterium phage genomes, Applejack, Hermia, LilBib, and QTRlifeCrisis

**Authors:** Noah E. Adelman, Leah Cromarty, Sonja M. Francis, Vejune E. Griciute, Jason N. Hart, Abigail Hoffpauir, Ryan Koss, Ronit Kumar, Melisa N. Matonsi, Emma Morrison, Grace Paiement, Autumn Perley, Adrian Plichta, Liliana Rodrigues, Melody N. Neely, Caitlin S. Wiafe-Kwakye, Sally D. Molloy

PMC · DOI: 10.1128/mra.00244-25 · Microbiology Resource Announcements · 2025-09-26

## TL;DR

This paper describes four new Mycobacterium phage genomes and their unique genetic features.

## Contribution

The study characterizes four new cluster A1 mycobacteriophages with unique enzymatic features.

## Key findings

- Four new siphoviruses (Applejack, Hermia, LilBib, QTRlifeCrisis) were isolated and sequenced.
- Applejack and LilBib encode metallophosphatases with inteins.
- Applejack contains a DNA methylase with an out-of-frame HNH endonuclease.

## Abstract

Four novel cluster A1 siphoviruses, Applejack, Hermia, LilBib, and QTRlifeCrisis, were isolated on Mycobacterium smegmatis. Their complete genome sequences are 49,633–50,325 bp in length and encode 80–91 protein-coding genes. Applejack and LilBib encode metallophosphatases containing inteins, and Applejack encodes a DNA methylase that contains an out-of-frame HNH endonuclease.

## Full-text entities

- **Species:** Mycolicibacterium smegmatis (species) [taxon 1772]

## Full text

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## Figures

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## References

24 references — full list in the complete paper: https://tomesphere.com/paper/PMC12613952/full.md

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Source: https://tomesphere.com/paper/PMC12613952