# Genome sequence of a human monkeypox virus isolate from Central Europe during the 2022 outbreak

**Authors:** Ármin Gergely Nagy, Ágota Ábrahám, István Prazsák, Balázs Kakuk, Brigitta Zana, Ágnes Nagy, Dóra Tombácz, Gábor Kemenesi, Zsolt Boldogkői

PMC · DOI: 10.1128/mra.00415-25 · Microbiology Resource Announcements · 2025-10-21

## TL;DR

This paper presents the genome sequence of a monkeypox virus from a Central European case in 2022, providing insights into its genetic makeup and classification.

## Contribution

The study provides a new genome sequence of a human monkeypox virus isolate from Central Europe during the 2022 outbreak.

## Key findings

- The genome is 197,993 bp long with a GC content of 32.9%.
- The isolate belongs to subclade IIb B.1.3.

## Abstract

We report the genome sequence of a human monkeypox virus isolated from an early Central European case. The 197,993 bp genome was assembled from amplicons sequenced using the Oxford Nanopore method. It has a GC content of 32.9% and belongs to subclade IIb B.1.3.

## Linked entities

- **Diseases:** monkeypox (MONDO:0002594)
- **Species:** Homo sapiens (taxon 9606)

## Full-text entities

- **Diseases:** smallpox (MESH:D012899), skin lesion (MESH:D012871)
- **Species:** Variola virus (smallpox virus, no rank) [taxon 10255], Monkeypox virus (no rank) [taxon 10244], Homo sapiens (human, species) [taxon 9606]
- **Cell lines:** Vero — Chlorocebus sabaeus (Green monkey), Spontaneously immortalized cell line (CVCL_0059)

## Full text

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## References

13 references — full list in the complete paper: https://tomesphere.com/paper/PMC12613930/full.md

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Source: https://tomesphere.com/paper/PMC12613930