# Whole-genome sequencing and determination of the properties of 18 strains of the genus Bacillus isolated from permafrost in Yakutia

**Authors:** Yulia Goncharova, Kseniya Khlopova, Vera Evseeva, Irina Bakhteeva, Raisa Mironova, Yuriy Skryabin, Angelina Kislichkina, Angelika Sizova, Ivan Dyatlov, Vitalii Timofeev

PMC · DOI: 10.1128/mra.00751-25 · Microbiology Resource Announcements · 2025-10-22

## TL;DR

This paper reports the whole-genome sequencing of 18 Bacillus strains from permafrost, revealing unique genetic and phenotypic traits.

## Contribution

The study identifies novel MLST sequence types and atypical properties in non-anthracis Bacillus strains from permafrost.

## Key findings

- Most strains belong to non-anthracis species of the Bacillus cereus complex.
- Some strains show sensitivity to anthrax bacteriophages and lack hemolytic activity.
- Unique MLST sequence types were identified among the isolates.

## Abstract

Here, we describe 18 strains of bacilli isolated from permafrost. Most strains belong to non-anthracis species of the Bacillus cereus complex. Some strains exhibit atypical properties such as sensitivity to anthrax bacteriophages and lack of hemolytic activity and have unique MLST sequence types.

## Linked entities

- **Species:** Bacillus cereus (taxon 1396), Bacillus (taxon 1386)

## Full-text entities

- **Species:** Bacillus cereus (species) [taxon 1396], Bacillus anthracis (anthrax bacterium, species) [taxon 1392]

## Full text

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## References

10 references — full list in the complete paper: https://tomesphere.com/paper/PMC12613914/full.md

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Source: https://tomesphere.com/paper/PMC12613914