# Draft genome sequence of Bordetella pertussis PS21, a circulating clinical isolate from India

## TL;DR

This paper presents the draft genome sequence of a Bordetella pertussis strain from India, highlighting mutations that may affect vaccine recognition.

## Contribution

The study provides a new genome sequence of a clinical B. pertussis isolate from India with specific mutations that could impact virulence and vaccine efficacy.

## Key findings

- The genome contains classical virulence genes and notable polymorphisms in key toxin and regulatory genes.
- Mutations in ptxA, bvgS, bapC, and ptxB may influence toxin function and immune recognition.
- These findings could inform understanding of B. pertussis evolution and vaccine effectiveness in India.

## Abstract

We report the draft genome sequence of Bordetella pertussis PS21, a clinical isolate obtained from a culture-positive sample from India. The 3.6 Mb genome harbors classical virulence genes and several notable polymorphisms, including a dual substitution in ptxA (Arg5Trp, Met228Ile), a Lys705Glu mutation in bvgS, a Lys97 frameshift in bapC, and a Gly45Ser substitution in ptxB. These mutations may influence pertussis toxin function and virulence regulation, potentially impacting immune recognition by pertussis vaccines.