# The Next Dimension: Digital Holography for 3D Interferometric Scattering

## TL;DR

This paper explains how digital holography can be used to improve the accuracy of measuring and tracking tiny particles in 3D.

## Contribution

The paper introduces a detailed experimental framework for using off-axis digital holography in high-sensitivity nanosizing applications.

## Key findings

- Off-axis digital holography offers advantages over traditional methods for interferometric scattering.
- Digital postprocessing enables 3D particle tracking and aberration correction.
- The approach is broadly applicable to nanosizing and particle characterization.

## Abstract

We provide detailed
experimental guidelines for implementing
digital
holography in the context of high-sensitivity interferometric scattering
(iSCAT)-based nanosizing applications. Our approach relies on interferometry
via the highly versatile off-axis implementation of digital holography,
which offers key advantages over more traditional strategies. After
a brief theoretical discussion of off-axis holography and its differences
and similarities with iSCAT, typical experimental implementations
and digital data-processing steps are presented. Key experimental
parameters and strategies to achieve optimal performance are also
highlighted. Following these experimental aspects, we focus on digital
postprocessing routines that enable digital refocusing and 3D particle
tracking as well as pupil function aberration correction. We then
conclude with a few examples highlighting the broad applicability
of digital holography for nanosizing and particle characterization
applications, as well as an outlook for future applications.