# Exploring the vector potential of Lipoptena cervi (Diptera: Hippoboscidae): first record of Setaria cervi (Filarioidea: Onchocercidae) DNA in deer keds

## TL;DR

This study found evidence that deer keds may carry a parasitic worm, suggesting they could help spread it between animals.

## Contribution

First molecular evidence of Setaria cervi DNA in Lipoptena cervi deer keds.

## Key findings

- Two L. cervi from red deer in eastern Slovakia carried S. cervi DNA.
- One red deer liver sample also tested positive for S. cervi DNA.

## Abstract

Setaria cervi is a filarial nematode that infects both wild and domestic ungulates. It is primarily transmitted by mosquitoes, although the possible role of other hematophagous insects is still uncertain. We investigated 83 wingless deer keds (Lipoptena spp.) from red and fallow deer in northern and eastern Slovakia, as well as 43 red deer liver samples for the presence of filarial DNA. Deer keds were identified as Lipoptena cervi (n = 80) and Lipoptena fortisetosa (n = 3). Genomic DNA from individual ectoparasites was screened by PCR targeting a mitochondrial cox1 gene fragment of filaroid nematodes. Two L. cervi from red deer in Hrabušice (eastern Slovakia) tested positive for S. cervi DNA, with 100% sequence identity with worms recently isolated from Slovak game animals. Additionally, S. cervi DNA was detected in one liver from a red deer in the Vranov nad Topl’ou district. This study provides the first molecular evidence of S. cervi DNA in L. cervi, suggesting a potential role of deer keds in the transmission at the wildlife-livestock-vector interface.

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•First detection of Setaria cervi DNA in Lipoptena cervi deer keds.•Two L. cervi from red deer in eastern Slovakia carried S. cervi DNA.•One red deer liver sample also tested positive for S. cervi DNA.•The findings suggest deer keds may play a role in filarioid transmission.•The study underscores the need for One Health surveillance of wildlife parasites.

First detection of Setaria cervi DNA in Lipoptena cervi deer keds.

Two L. cervi from red deer in eastern Slovakia carried S. cervi DNA.

One red deer liver sample also tested positive for S. cervi DNA.

The findings suggest deer keds may play a role in filarioid transmission.

The study underscores the need for One Health surveillance of wildlife parasites.