# Effect of Torrefaction Condensate on the Growth and Exopolysaccharide Production of Chlamydomonas reinhardtii

## TL;DR

This study explores how torrefaction condensate affects the growth and exopolysaccharide production of the green microalgae Chlamydomonas reinhardtii.

## Contribution

The study introduces a novel biorefinery approach using torrefaction condensate to produce value-added exopolysaccharides from microalgae.

## Key findings

- Torrefaction condensate at 2 mL/L and 2.5 mL/L concentrations negatively impacts growth and EPS production in C. reinhardtii.
- C. reinhardtii adapts to torrefaction condensate over time, showing growth patterns similar to the control.
- EPS produced in torrefaction condensate has distinct biochemical and antioxidant properties compared to the control.

## Abstract

Torrefaction, a mild thermochemical pretreatment process, generates the fuel-torrefied biomass along with non-condensable and condensable gases. The latter can be condensed to yield a dark, viscous liquid called torrefaction condensate (TC). In this study, we investigated the effect of TC on growth and exopolysaccharide (EPS) production by the green microalgae Chlamydomonas reinhardtii, a well-known model organism. Aspen wood pellets were torrefied at different temperatures, and the condensate formed at each temperature was analyzed. Based on the GC-MS analysis, 225 °C TC was selected and used for the cultivation of C. reinhardtii. Results show that at 2 mL/L and 2.5 mL/L concentrations, TC negatively impacts growth, EPS production, as well as the composition of amino acids, lipids, and fatty acids n of C. reinhardtii. However, C. reinhardtii gradually adapted to TC and attained the growth patterns comparable to the control, showing the resilience of the culture. The biochemical and antioxidant properties of the EPS showed significant differences to that of the control. Therefore, cultivating these microalgae in TC suggests a new microalgal biorefinery approach through the utilization of low-value TC for the production of value-added products, such as EPS.