# Seaweed Foliar Biostimulants Improve Growth and Phytochemicals of Thai Basil (Ocimum basilicum L.) in a Plant Factory

## TL;DR

This study shows that using seaweed extract as a foliar spray improves the growth and health benefits of Thai basil plants in a controlled environment.

## Contribution

The study identifies the optimal concentration of seaweed extract for maximizing growth and phytochemical production in Thai basil.

## Key findings

- Moderate concentrations of seaweed extract (1.0–2.0 mL·L−1) significantly improved plant growth and chlorophyll levels.
- The 2.0 mL·L−1 treatment increased total phenolic content by 1.88-fold compared to the control.
- Seaweed extract treatments enhanced rosmarinic acid accumulation and activated the phenylpropanoid pathway.

## Abstract

This study aimed to identify the optimal concentration of seaweed extract (SE) for enhancing growth, photosynthetic traits, antioxidant activity, and bioactive compound accumulation in Thai basil (Ocimum basilicum L.) plants cultivated in a fully controlled plant factory. Basil plants were foliar-sprayed twice weekly with five SE concentrations (0.5, 1.0, 1.5, 2.0, and 2.5 mL·L−1), while untreated plants served as controls. After 28 days of transplanting, plant growth parameters, photosynthetic parameters, chlorophyll pigments, antioxidant activity, and the concentrations of phenolic acids and rosmarinic acid (RA) were analyzed. Moderate SE concentrations (1.0–2.0 mL·L−1) significantly enhanced plant growth, chlorophyll a, carotenoid levels, DPPH radical scavenging, and total flavonoid content relative to control. The 2.0 mL·L−1 treatment produced the highest total phenolic content (1.88-fold increase over the control) and was associated with elevated benzoic acid, rutin, quercetin, and kaempferol, along with reduced trans-cinnamic acid, indicating activation of the phenylpropanoid pathway. Moreover, all SE treatments significantly increased RA accumulation. These findings demonstrate that SE is an effective, sustainable biostimulant for Thai basil, with 2.0 mL·L−1 as the optimal concentration for maximizing growth and phytochemical production.