# Enhancement of Pea–Oat Composite Protein Gel Properties Through Ultrasound Treatment Affects Structural and Functional Characteristics

## TL;DR

This study shows that ultrasound treatment improves the structure and function of pea-oat protein gels, making them more elastic and thermally stable.

## Contribution

The novel use of ultrasound to enhance plant-based composite protein gels is demonstrated with structural and functional improvements.

## Key findings

- Ultrasonic treatment at 400 W for 30 min improved water-holding capacity, gel strength, and surface hydrophobicity of POPG.
- Rheological analysis showed increased storage modulus and viscoelasticity in treated POPG samples.
- Ultrasonication increased β-sheet content and thermal stability, resulting in a more compact and firm gel structure.

## Abstract

With increasing attention to health, plant protein products have gained significant market potential due to their growing consumer demand. This study researches the influence of ultrasonic treatment on the structure and function of pea–oat composite protein gel (POPG) to enhance its elasticity and thermal stability. The ultrasonic treatment parameters were regulated to power (200–600 W for 30 min) and ultrasonic time (20–40 min at 400 W) during the preparation of POPG, and the properties and structure, including gel strength, rheological analysis, water-holding capacity (WHC), thermal characteristics, fluorescence performance, and microstructure, were further evaluated. The results showed that the POPG samples exhibited optimal values in WHC, gel strength, surface hydrophobicity, free sulfhydryl amount, and endogenous fluorescence at 400 W ultrasonic for 30 min compared with the untreated POPG. Rheological analysis indicated that POPG displayed the highest storage modulus and improved viscoelasticity. Ultrasonication resulted in an augmentation in β-sheet content, hence creating a more compact network structure. DSC and TGA revealed improved thermal stability, while SEM and CLSM exhibited a homogeneous and firm gel structure of POPG. This research offers the theory that ultrasonic technology can improve the performance of plant-based composite gels.