# Exploring the Antidiabetic Properties of Polyalthia longifolia Leaf and Stem Extracts: In Vitro α-Glucosidase and Glycation Inhibition

## TL;DR

This study explores how extracts from Polyalthia longifolia leaves and stems may help manage diabetes by inhibiting enzymes and harmful chemical reactions linked to high blood sugar.

## Contribution

The study identifies ethanol and methanol leaf extracts of P. longifolia as having significant antidiabetic potential through in vitro inhibition of α-glucosidase and glycation.

## Key findings

- Ethanol and methanol extracts from P. longifolia leaves showed significant antidiabetic activity.
- Phytochemical analysis identified phenolic compounds and flavonoids as key bioactive components.
- Extracts exhibited antiglycation and antiradical properties, with slight cytotoxicity observed in HT-29 cells.

## Abstract

Polyalthia longifolia, a member of the Annonaceae family, is traditionally used for its medicinal properties, including as an antidiabetic remedy, primarily in Asia and sub-Saharan Africa. This study investigated the potential of six P. longifolia extracts in counteracting hyperglycemia and diabetes-related complications. Aqueous, ethanol, and methanol extracts from leaves and stems were evaluated for their antihyperglycemic, antiglycation, and antiradical properties using α-glucosidase, BSA, and ORAC assays, respectively. Phytochemical characterization was conducted using TPC and TFC assays, and HPLC analysis identified specific bioactive compounds, including various phenolic compounds (gallic acid, (+)-catechin, epicatechin, caffeic acid, ellagic acid and rosmarinic acid) and flavonoids (luteolin, kaempferol and baicalein). The MTT assay on the human cell line HT-29 assessed the activity of extracts on cell viability, showing slight cytotoxicity. Results demonstrated significant antidiabetic activity of the ethanol and methanol extracts from P. longifolia leaves. This study provides new insights into the potential use of P. longifolia in diabetes mellitus and supports the valorization of traditional medicinal plants.