Mechanisms Underlying the Cognitive Benefits of Solanum macrocarpon Leaf n-Butanol Extract: Acetylcholinesterase Inhibition and Oxidative Stress Modulation
Ion Brinza, Ibukun Oluwabukola Oresanya, Ilkay Erdogan Orhan, Hasya Nazlı Gök, Lucian Hritcu, Razvan Stefan Boiangiu
TL;DR
This study shows that an extract from Solanum macrocarpon leaves helps reduce anxiety and cognitive issues in zebrafish by improving brain function and reducing oxidative stress.
Contribution
The study identifies acetylcholinesterase inhibition and antioxidant effects as mechanisms for the cognitive benefits of Solanum macrocarpon leaf extract.
Findings
SMB reduced anxiety-like behaviors and cognitive deficits in a dose-dependent manner in zebrafish.
SMB inhibited acetylcholinesterase activity and enhanced antioxidant enzyme activity.
SMB lowered oxidative stress biomarkers like malondialdehyde and protein carbonyls.
Abstract
This study investigates the neuroprotective and anxiolytic effects of Solanum macrocarpon L. leaf n-butanol extract (SMB) in a zebrafish model of scopolamine (SCOP; 100 μM)-induced cognitive and behavioral impairments. SCOP, a muscarinic receptor antagonist, is commonly used to mimic memory deficits and anxiety-like behaviors associated with neurodegenerative conditions. Zebrafish were chronically exposed to SMB at concentrations of 1, 3, and 6 mg/L. Behavioral assessments included anxiety-related paradigms, such as novel tank diving (NTT), novel approach (NA), and light–dark transition (LD) tests, as well as cognitive assays, including the Y-maze and novel object recognition (NOR) tests. SMB significantly mitigated SCOP-induced anxiety-like behaviors and cognitive deficits in a dose-dependent manner. Biochemical analyses demonstrated that SMB inhibited acetylcholinesterase (AChE)…
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Taxonomy
TopicsZebrafish Biomedical Research Applications · Marine Toxins and Detection Methods · Cholinesterase and Neurodegenerative Diseases