# The Effect of Bilayered Bioactive Coating on Polycaprolactone Electrospun Scaffold Biocompatibility, Bioabsorption and Cellular Properties

## TL;DR

A new bioactive coating improves the performance of polycaprolactone scaffolds for tissue engineering by enhancing cell attachment and reducing inflammation.

## Contribution

A bilayered bioactive coating with heparin and platelet lysate is introduced to enhance scaffold biocompatibility and cellular properties.

## Key findings

- The bioactive coating does not alter scaffold structure or mechanical properties but improves cell adhesion and proliferation.
- Modified scaffolds showed higher cell density (185.6 ± 15.7 cells/mm²) compared to controls (79.5 ± 7.8 cells/mm²).
- The coating reduces inflammation and accelerates vascularization in vivo.

## Abstract

Bioabsorbable scaffolds from synthetic polyesters are widely used in the field of tissue engineering. However, their hydrophobic surface and lack of suitable functional groups are the main limitations related to cell attachment. The aim of this research was to modify the surface of polycaprolactone (PCL) scaffolds using a bioactive coating containing heparin bound via albumin spacer and platelet lysate over heparin. Porous scaffolds were produced by electrospinning from 10% PCL (w/w) solution in methylene chloride (25 kV voltage, 100 mm distance between electrodes and 4 mL/h feedrate), which demonstrated 5.5 ± 1.1 MPa Young’s modulus, 2.5 ± 0.4 MPa tensile strength and 321 ± 29% elongation at break. Bioactive coating does not change the structure and mechanical properties of the scaffolds. Treated scaffolds are biocompatible and have no cytotoxic effect in direct contact with cells. Functionalization also promotes the in vitro adhesion and proliferation of human adipose mesenchymal stromal cells. After 7 days of incubation, the PCL scaffold modified with the heparin–platelet lysate complex had a cell density of 185.6 ± 15.7 cells/mm2 compared to 79.5 ± 7.8 cells/mm2 for nontreated control. The intramuscular implantation of scaffolds revealed that immobilization of heparin alone prolongs the acute phase of the inflammatory reaction. However, subsequent treatment with platelet lysate minimizes the inflammatory reaction, slows the rate of implant absorption, and accelerates vascularization. The results obtained show that the developed bioactive coating improves the cellular properties of PCL electrospun scaffolds and can be used to form in vivo tissue-engineered constructs.