# Low-Cost Versatile Microfluidic Platform for Bioorthogonal Click-Mediated Nanoassembly of Hybrid Nanosystems

## TL;DR

This paper introduces a low-cost microfluidic platform for efficiently modifying nanoparticles using click chemistry, suitable for biomedical applications.

## Contribution

The novel contribution is an ultra-low-cost microfluidic platform for versatile nanoparticle modification using bioorthogonal click chemistry.

## Key findings

- The platform successfully modified both soft and rigid nanoparticles with small molecules and other nanoparticles.
- The method uses click chemistry, making it compatible with industrial drug development standards.
- The approach demonstrates versatility and reproducibility for nanomedicine production.

## Abstract

In recent years the global market of nanomedicine has experienced incredible growth owing to the advances in the field. This translation of the technique to the biomedical industry requires the development of production methods that deliver nanomedicines with a high degree of reproducibility between batches, combined with cost and time efficiency. The use of nanoparticles in medicine usually requires their surface functionalization to improve biocompatibility in addition to providing targeting capacities and/or stimuli-responsive behavior, among other interesting skills. Microfluidic technology has revolutionized the field both in nanomedicine synthesis and in preclinical evaluation. However, microfluidic-assisted synthetic procedures commonly require high-cost methods and equipment to fabricate the microreactors. The aim of this work is to present an ultra-low-cost microfluidic platform that permits the versatile modification of nanomaterials. To prove this approach, two different model nanoparticles with different natures: soft nanoparticles (liposomes) and rigid nanoparticles (mesoporous silica) have been decorated both with small molecules and with other nanoparticles, respectively, in order to evaluate the scope of this approach. The anchoring of the covalently attached elements has been performed using click chemistry, in compliance with the principles for further transfer to the drug industry.