# Research on Geographical Origin Traceability of Salvia miltiorrhiza by Combining Two-Trace Two-Dimensional (2T2D) Correlation Spectroscopy and Improved DeiT Model

**Authors:** Jinpo Yang, Kai Chen, Yimin Zhou, Jian Zheng, Linhao Sun, Yun Zhang, Zhu Zhou

PMC · DOI: 10.3390/plants14213365 · Plants · 2025-11-03

## TL;DR

This paper introduces a new method using advanced spectroscopy and deep learning to accurately trace the geographical origin of Salvia miltiorrhiza, helping combat counterfeit products.

## Contribution

The novel integration of 2T2D correlation spectroscopy with an improved DeiT-CBAM model enables high-accuracy traceability of Salvia miltiorrhiza origin.

## Key findings

- 2T2D correlation spectroscopy images outperformed traditional one-dimensional spectral models in authenticity and origin identification.
- The DeiT-CBAM model achieved 100% accuracy on training and validation sets and 99.62% on the test set using only 79 wavelengths.
- Layer-wise CAM validated the model's interpretability, confirming its robust performance for geographical traceability.

## Abstract

Salvia miltiorrhiza Bunge (Danshen) is widely used in modern medicine, but the market faces challenges from counterfeit and mislabeled geographical indication products. To address this, we propose a novel framework combining Two-trace Two-dimensional (2T2D) correlation spectroscopy, hyperspectral imaging (HSI), transfer learning, and an enhanced deep learning model (DeiT-CBAM) to identify both authenticity and origin precisely. Hyperspectral data (873–1720 nm) were collected from six genuine and three adulterated regions and converted into synchronous 2T2D correlation spectroscopy images. We systematically evaluated five preprocessing strategies, three wavelength selection methods, three classical models, and four deep learning models. Models based on 2T2D correlation spectroscopy images consistently outperformed traditional one-dimensional spectral models. Notably, the DeiT-CBAM model, integrated with the successive projections algorithm (SPA), achieved optimal performance using only 79 wavelengths, with 100% accuracy on the training and validation sets and 99.62% on the test set, without the need for additional preprocessing. Model interpretability was further validated through layer-wise class activation mapping (layer-wise CAM). This study demonstrates that the integration of synchronous 2T2D correlation spectroscopy images with the DeiT-CBAM model offers robust discriminative performance, providing a reliable technical solution for geographical origin traceability of food, medicinal herbs, and other species.

## Linked entities

- **Species:** Salvia miltiorrhiza (taxon 226208)

## Full-text entities

- **Chemicals:** Salvia miltiorrhiza Bunge (-)
- **Species:** Salvia miltiorrhiza (Chinese salvia, species) [taxon 226208]

## Full text

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## Figures

8 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12608159/full.md

## References

60 references — full list in the complete paper: https://tomesphere.com/paper/PMC12608159/full.md

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Source: https://tomesphere.com/paper/PMC12608159