# Differential Expression of Circular RNAs in Rat Brain Regions with Various Degrees of Damage After Ischemia–Reperfusion

## TL;DR

This study explores how circular RNAs change in rat brain regions with different levels of damage after a stroke-like event, suggesting their role in brain recovery.

## Contribution

The study identifies differentially expressed circRNAs in brain regions with varying ischemic injury and reveals their potential roles in regulating inflammation and recovery.

## Key findings

- 597 differentially expressed circRNAs were identified in the striatum after ischemia–reperfusion injury.
- DECs in the striatum and frontal cortex showed contrasting expression patterns, suggesting region-specific roles.
- DECs were linked to genes involved in inflammation and neurotransmission, indicating their regulatory functions in brain injury.

## Abstract

Circular RNAs (circRNAs) are non-coding RNAs that can significantly influence the regulation of gene expression in health and disease, including ischemic stroke. We identified 597 differentially expressed circRNAs (DECs) (fold change > 1.5; Padj < 0.05) in the striatum region encompassing the ischemic lesion and penumbra 24 h after ischemia–reperfusion injury (tMCAO) in rats, according to high-throughput RNA sequencing data (RNA-Seq). The DECs predominantly increased expression levels relative to those in sham-operated animals. In this study, we also compared these data with DECs we previously identified in the frontal cortex region containing the penumbra and healthy tissue. Furthermore, we bioinformatically constructed a network of competitive circRNA-microRNA-mRNA interactions characterizing the possible functions of DECs in brain areas with varying degrees of ischemic injury. We found that in both tissues, the identified DECs were involved in regulating the expression of genes associated with inflammation and neurotransmission. Moreover, in the striatum, most DECs decreased their expression, while in the frontal cortex, most DECs increased their expression. Thus, we demonstrated different circRNA activities in brain areas with varying degrees of injury. This result may indicate a role for these molecules in regulating brain cell responses, including those important for functional recovery after cerebral ischemia.