# miR-28-5p and miR-708-5p Share a Common Seed with Different Functions in Lung Cancer Patients

## TL;DR

This study explores miR-28-5p and miR-708-5p in lung cancer, finding that they share a seed sequence but have different roles, with miR-708-5p showing potential for clinical use.

## Contribution

The study reveals distinct functional roles of two miRNAs with a shared seed sequence in lung cancer.

## Key findings

- miR-28-5p and miR-708-5p are overexpressed in advanced-stage lung cancer tissues.
- miR-708-5p shows consistent overexpression and potential diagnostic value in lung cancer.
- Both miRNAs regulate cancer-related pathways like ECM-receptor interaction and Hippo signaling.

## Abstract

Lung cancer remains the leading cause of cancer-related mortality worldwide, accounting for nearly 1.8 million deaths annually. The present study aimed to investigate the role of miR-28-5p and miR-708-5p in lung cancer and to analyze the relationship between target gene profiles and transcriptional factor regulation. Both miRNAs that share a common seed sequence were found to be overexpressed in a cohort of 32 paired tumor and adjacent normal tissue samples collected from patients diagnosed at advanced stages (III and IV) of disease. Data from the dbDEMC database revealed that miR-28-5p exhibited variable expression across lung cancer subtypes, whereas miR-708-5p showed consistent overexpression, reinforcing its potential clinical diagnostic significance. Using the TransmiR database, we identified complex TF–miRNA regulatory networks, with both shared and distinct transcription factors controlling miR-28-5p and miR-708-5p. Pathway enrichment analysis indicated that these miRNAs regulate several cancer-associated pathways, including ECM–receptor interaction, adherens junctions, and Hippo signaling. Overall, our findings suggest that miR-708-5p may have a potential clinical application in lung cancer.