# An L-shaped flexible neural implant for chronic ECoG signal acquisition in M2 region of control and Parkinsonian rat models

## TL;DR

A new L-shaped neural implant is developed to record brain signals from specific areas in rat models of Parkinson's disease.

## Contribution

The novel L-shaped implant is designed for targeted chronic ECoG signal acquisition in specific rodent brain regions.

## Key findings

- The L-shaped implant with five electrodes was successfully fabricated using biocompatible Polyimide material.
- ECoG signals were acquired for two weeks in control and hemi-parkinsonian rats using the new implant.
- The implant's average electrode impedance was measured at 18.315 kΩ at 1 kHz.

## Abstract

Neural implants help understand neurological disorders and are actively used to study deep and cortical brain surface regions. Dealing with cortical surface regions is less complicated in clinical therapy than deep brain regions. Researchers are interested in identifying cortical surface region/s for a particular neurological disorder. Rodent models are extensively used in preclinical studies. Usually, microwires, screws, and grid-type implants are used for such studies, but they are not designed for specific rodent brain regions. Since the grids are typically standard in size, in some cases, the craniotomy required to implant the grid will be significantly bigger than the region of interest, which may pose challenges for chronic studies due to infection. Additionally, the grids may block the nearby brain regions in multisite studies, posing difficulty for another device to be implanted. In this study, a novel L-shaped surface neural implant with five electrodes (diameter: 400 μm) spanning a 1 mm × 3 mm area is fabricated using biocompatible Polyimide material for cortical surface studies. The overall thickness of the neural implant is around 25 μm. The average impedance of the electrodes is 18.315 kΩ at 1 kHz. A bilateral craniotomy is performed to place the neural implants in the secondary motor area for subdural chronic electrocorticography (ECoG) signal acquisition in control and hemi parkinsonian rats. After the recovery period, the ECoG signals are acquired using the Open BCI Cyton Daisy Biosensing board for two weeks from the rats.

The online version contains supplementary material available at 10.1038/s41598-025-23049-0.