# A farnesoid X receptor T296I variant disrupts ligand-induced FXR activation and thus bile acid transport in progressive familial intrahepatic cholestasis

## TL;DR

A genetic variant in the FXR protein disrupts bile acid transport and causes a rare liver disease, offering insights for new treatments.

## Contribution

First study to describe the conformational change of FXR LBD and its disruption by a T296I variant in PFIC.

## Key findings

- The T296I variant reduces FXR transcriptional activity on BSEP and SHP in patient-derived models.
- BSEP-dependent bile acid transport is impaired in T296I organoids but can be rescued with FXR WT mRNA.
- Molecular dynamics show reduced transition to the active state in the T296I FXR variant.

## Abstract

Nuclear receptor farnesoid X receptor (FXR) acts as a key regulator of bile acid pool homeostasis and metabolism. Within the enterohepatic circulation, reabsorbed bile acids act as FXR agonists. FXR, in turn, transcriptionally controls the synthesis and transport of bile acids. Binding occurs in the ligand binding domain (LBD), favoring a conformational change to the active state in which helix 12 interacts with the LBD to form an interaction surface for nuclear co-activators. The homozygous missense variant T296I, identified in a patient with progressive familial intrahepatic cholestasis (PFIC), is located close to the critical helix 12 interaction. Here, we identified reduced transcriptional activity of the variant protein on the downstream targets bile salt export pump (BSEP) and small heterodimer partner (SHP) in vitro, within the patient's liver, and in iPSC-derived hepatic organoids. BSEP-dependent Tauro-DBD transport was impaired in T296I patient-derived organoids, but could be rescued via lipid nanoparticle-mediated FXR WT mRNA delivery, indicating the variant is responsible for the identified reduced BSEP expression. Using molecular dynamics simulations, we observed a reduced transition from the inactive to the active state for the T296I variant, indicating a molecular mechanism underlying the reduced activity. To our knowledge, this is the first study to describe the conformational change from an inactive to an active state of the FXR LBD. This might be useful for new therapeutic approaches targeting the activation of FXR.