# Recent Advances in Laccase Production: Challenges and Future Perspectives **Authors:** Mingxia Jiao, Wenhu Chen, Xiangling Jiang, Di Huang, Yi Jiang, Hongling Liu, Haibo Yuan, Tengfei Wang PMC · DOI: 10.4014/jmb.2507.07052 · Journal of Microbiology and Biotechnology · 2025-10-27 ## TL;DR This review discusses recent progress in laccase production, highlighting its benefits and potential in various industries. ## Contribution The paper provides a comprehensive overview of laccase improvement strategies and future research directions. ## Key findings - Laccase has broad industrial applications due to its catalytic properties. - Recent studies focus on enhancing laccase production efficiency. - Future research trends aim to optimize laccase for industrial use. ## Abstract Laccase, a type of multicopper oxidase, exhibits several notable advantages, including broad distribution, wide catalytic scope, and high oxidation-reduction potential. Owing to its enormous potential in food, textiles, paper, chemical synthesis, and bioremediation, laccase is considered a green catalyst in wide-ranging industrial applications. To aid in understanding the current developments in laccase research, this review introduces the physiological functions, classification, and reaction mechanisms of laccase, with a focus on improvement strategies. In addition, the updated studies and advancements in laccase production are summarized. Finally, the possible research trends and general development directions for more efficient laccase production are also proposed. ## Linked entities - **Proteins:** LOC7454935 (laccase-2) ## Full-text entities - **Diseases:** toxicity (MESH:D064420), inflammatory compounds (MESH:D005597), endocrine disruptor (MESH:D004700) - **Chemicals:** 1,8-dihydroxynaphthalene (MESH:C041660), phenol (MESH:D019800), guaiacol (MESH:D006139), epoxy (MESH:D004853), mannose (MESH:D008358), His (MESH:D006639), maltose (MESH:D008320), water (MESH:D014867), H2O2 (MESH:D006861), R-478 (MESH:C017971), ethyl methane sulfonate (MESH:D005020), alcohols (MESH:D000438), gallic acid (MESH:D005707), lignocellulose (MESH:C036909), glycans (MESH:D011134), hydrogen (MESH:D006859), xylose (MESH:D014994), Ethanol (MESH:D000431), microcrystalline cellulose (MESH:C109691), CuSO4 (MESH:D019327), syringaldazine (MESH:C009370), CNTs (MESH:D037742), free radicals (MESH:D005609), polymer (MESH:D011108), amines (MESH:D000588), phenoxyl radicals (MESH:C042329), penicillin (MESH:D010406), perfluorooctanoic acid (MESH:C023036), Tween 80 (MESH:D011136), anthraquinone (MESH:D000880), Carbon (MESH:D002244), phenols (MESH:D010636), starch (MESH:D013213), sucrose (MESH:D013395), copper (MESH:D003300), mercury (MESH:D008628), cellobiose (MESH:D002475), urea (MESH:D014508), reactive blue 19 (MESH:C024758), glycerol (MESH:D005990), carbohydrate (MESH:D002241), O2 (MESH:D010100), carboxyl radicals (MESH:C041069), cysteine (MESH:D003545), 1,3,8-trihydroxynaphthalene (MESH:C522206), polyphenols (MESH:D059808), 1-hydroxybenzotriazole (MESH:C011852), p-coumaric acid (MESH:C495469), 5-chloro-2-(2,4-dichlorophenoxy) phenol (MESH:D014260), (+-)-dalesconols A, B, and C (-), fructose (MESH:D005632), T1 (MESH:C103828), chlorine (MESH:D002713), metal (MESH:D008670), Bisphenol A (MESH:C006780), lignin (MESH:D008031), benzenethiols (MESH:C042983), cephalosporins (MESH:D002511), galactose (MESH:D005690), 2,5-dihydroxybenzoic acid (MESH:C010925) - **Species:** Trametes pubescens (species) [taxon 154538], Shouchella clausii (species) [taxon 79880], Komagataella pastoris (species) [taxon 4922], Panus rudis (species) [taxon 48022], Streptomyces coelicolor (species) [taxon 1902], Lentinus tigrinus (species) [taxon 5365], Pleurotus ostreatus (oyster mushroom, species) [taxon 5322], Geobacter metallireducens (species) [taxon 28232], Cyathus bulleri (species) [taxon 184115], Gelatoporia subvermispora (species) [taxon 42742], Kluyveromyces sp. (species) [taxon 1982016], Anthracophyllum (genus) [taxon 182043], Escherichia coli (E. coli, species) [taxon 562], PX clade (clade) [taxon 569578], T. pubescens [taxon 622621], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Thermus thermophilus HB27 (strain) [taxon 262724], Arachis hypogaea (goober, species) [taxon 3818], Penaeus vannamei (Pacific white shrimp, species) [taxon 6689], Heterotermes crinitus (species) [taxon 1367271], Komagataella phaffii GS115 (strain) [taxon 644223], Melanocarpus albomyces (species) [taxon 204285], Helianthus annuus (common sunflower, species) [taxon 4232], Pycnoporus sp. (in: basidiomycete fungi) (species) [taxon 2047320], Lentinus crinitus (species) [taxon 5358], Azospirillum lipoferum (species) [taxon 193], Borassus flabellifer (species) [taxon 145675], Sclerotinia sclerotiorum (species) [taxon 5180], Pichia sp. (species) [taxon 4925], Trametes hirsuta (species) [taxon 5327], Trametes versicolor (turkey-tail fungus, species) [taxon 5325], Cerrena unicolor (species) [taxon 90312], Ganoderma lucidum (species) [taxon 5315], Musa acuminata (banana, species) [taxon 4641], Streptomyces griseorubens (species) [taxon 66897], Solanum lycopersicum (tomato, species) [taxon 4081], Albifimbria verrucaria (species) [taxon 1859699], Bacillus sp. (in: firmicutes) (species) [taxon 1409], C. unicolor [taxon 125874], Toxicodendron vernicifluum (Chinese lacquer, species) [taxon 4013], [Candida] sp. (species) [taxon 1853550], Daldinia eschscholzii IFB-TL01 (strain) [taxon 1169046], Bacillus subtilis (species) [taxon 1423], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Gongronella sp. (species) [taxon 1981611] - **Mutations:** C-92 C, Y230R, His-cysteine, A248D, L from 12,000, L in 8 - **Cell lines:** AH28-2 — Homo sapiens (Human), Induced pluripotent stem cell (CVCL_A8MQ), SYBC-L3 — Homo sapiens (Human), Embryonic stem cell (CVCL_ZJ90) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12603382/full.md ## Figures 6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12603382/full.md ## References 220 references — full list in the complete paper: https://tomesphere.com/paper/PMC12603382/full.md --- Source: https://tomesphere.com/paper/PMC12603382