# Effects of Eisenia bicyclis Extract on Sleep Promotion in a Caffeine-Induced Insomnia Models

## TL;DR

This study shows that Eisenia bicyclis extract helps reduce insomnia caused by caffeine in fruit flies and mice by improving sleep quality and reducing brain damage.

## Contribution

The study demonstrates the novel sleep-promoting effects of Eisenia bicyclis extract in caffeine-induced insomnia models.

## Key findings

- EB treatment normalized disrupted sleep patterns and reduced sleep fragmentation in caffeine-exposed fruit flies.
- EB reduced caffeine-induced hyperactivity and brain damage by enhancing antioxidant defenses and neurotransmitter levels.
- EB restored caffeine-reduced sleep duration in mice without affecting sleep latency.

## Abstract

This study investigated the sleep-promoting effects of the brown alga Eisenia bicyclis (EB), which contains phlorotannins, using caffeine-induced insomnia models of Drosophila melanogaster and ICR mice. In flies exposed to caffeine, EB treatment dose-dependently normalized disrupted nighttime activity and total sleep duration, while high-dose EB significantly reduced sleep fragmentation by decreasing the number of sleep bouts. Locomotor tracking analysis further showed that EB attenuated caffeine-induced hyperactivity, reducing distance moved, velocity, and mobility to levels comparable with the normal and benzodiazepine (BDZ)-treated groups. In the pentobarbital-induced sleep test with mice, EB restored the caffeine-induced reduction in sleep duration, although sleep latency remained unaffected. Moreover, EB significantly reduced elevated brain malondialdehyde levels induced by caffeine, accompanied by increased expression of antioxidant-related enzymes. Neurochemical analyses revealed that EB enhanced the levels of γ-aminobutyric acid (GABA) and serotonin, as well as the expression of their receptors, effectively reversing caffeine-induced reductions. These findings suggest that EB exerts sleep-promoting effects by modulating behavioral activity, enhancing antioxidant defense, and regulating GABAergic and serotonergic neurotransmission. Collectively, our results support the potential application of EB as a marine algae-derived functional material with relevance for both the food and pharmaceutical industries in the management of sleep disorders.