# The complete chloroplast genome of Tinospora sinensis and its phylogenetic analysis

**Authors:** Jiahua Chen, Yang Ni, Guoan Shen, Chang Liu

PMC · DOI: 10.1080/23802359.2025.2582534 · Mitochondrial DNA. Part B, Resources · 2025-11-09

## TL;DR

This paper presents the full chloroplast genome of Tinospora sinensis, a medicinal plant, and explores its evolutionary relationships.

## Contribution

The first complete chloroplast genome assembly and phylogenetic analysis of Tinospora sinensis is reported.

## Key findings

- The chloroplast genome is 160,664 bp long with 126 genes and a GC content of 39.31%.
- 256 SSRs and 50 dispersed repeats were identified as potential molecular markers.
- Phylogenetic analysis confirmed close relationships with other Tinospora species.

## Abstract

Tinospora sinensis (Lour.) Merr. (Menispermaceae) is a medicinal plant with limited genomic resources available. Here, we assembled and annotated its 160,664 bp chloroplast genome, identifying 126 genes with a GC content of 39.31%. We detected 256 SSRs and 50 dispersed repeats, which represent potential molecular markers. IR boundary analysis showed slight expansions/contractions involving rps19, ndhF, and ycf1. Phylogenetic analysis confirmed its close evolutionary relationship with other Tinospora species. This is the first comprehensive genomic characterization of T. sinensis, providing valuable resources for taxonomy, evolution, and medicinal research in Menispermaceae.

## Linked entities

- **Genes:** RPS19 (ribosomal protein S19) [NCBI Gene 6223], ndhF (NADH dehydrogenase subunit 5) [NCBI Gene 800484], ycf1 (hypothetical chloroplast RF1) [NCBI Gene 800970]
- **Species:** Tinospora sinensis (taxon 152349), Mus musculus (taxon 10090)

## Full-text entities

- **Genes:** RPS19 (ribosomal protein S19) [NCBI Gene 6223] {aka DBA, DBA1, LOH19CR1, S19, eS19}
- **Species:** Tinospora sinensis (species) [taxon 152349]

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12599365/full.md

## References

36 references — full list in the complete paper: https://tomesphere.com/paper/PMC12599365/full.md

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Source: https://tomesphere.com/paper/PMC12599365