# An experimental approach to study foraging memory in ectomycorrhizal mycelium

## TL;DR

This study explores whether the fungus Laccaria bicolor can remember the location of a nutrient source through mycelial growth patterns.

## Contribution

The paper introduces a novel experimental framework to investigate memory-like behavior in ectomycorrhizal fungi.

## Key findings

- No evidence of memory was found in Laccaria bicolor mycelium when transferred to fresh medium.
- The developed methodology provides a basis for future studies on fungal behavior and cognition.

## Abstract

Behavioral ecology of fungi is an emerging field investigating how fungi respond to environmental stimuli through morphological and physiological changes. Progress requires methodologies suited to fungal biology. Here, we developed an experimental approach to test for memory in the ectomycorrhizal fungus Laccaria bicolor. We hypothesized that mycelium exposed to pea cotyledons would retain directional information about the nutrient source. To test this, a portion of the mycelium was transferred to fresh medium, where memory would be assessed by asymmetrical growth toward the former nutrient position. The hypothesis was not supported, but the methods offer a framework for exploring fungal behavior in both ectomycorrhizal and saprotrophic species. Although no evidence of memory was found, this study highlights the value of publishing both positive and negative results and provides tools to advance research on fungal cognition and behavior.