# Biocontrol-relevant diversity of wheat-associated Pseudomonas: prevalence of P. sivasensis and identification of the novel species P. arvensis sp. nov

## TL;DR

This study explores Pseudomonas bacteria in wheat and spelt, identifying new species and their potential to control plant diseases.

## Contribution

The discovery of Pseudomonas arvensis sp. nov. and the biocontrol potential of P. sivasensis in wheat.

## Key findings

- Pseudomonas sivasensis shows strong antagonistic activity against wheat pathogens and colonizes plants effectively.
- Pseudomonas arvensis sp. nov. is a newly identified species with potential biocontrol properties.
- Native Pseudomonas species may serve as sustainable biocontrol agents for cereal crops.

## Abstract

The role of microbial communities in plant health and productivity has become increasingly evident. In this study, we investigated the diversity and functional potential of Pseudomonas spp. associated with Belgian cereal crops, focusing on wheat and spelt. From 444 Pseudomonas isolates, we identified 11 species, with several strains exhibiting strong antagonistic activity against major wheat pathogens. Notably, we report the high prevalence and efficient colonization abilities of Pseudomonas sivasensis, including its capacity to translocate from seed to leaf and suppress Zymoseptoria tritici infection in planta. Furthermore, we describe Pseudomonas arvensis sp. nov., a newly characterized species within the wheat microbiome, with DR1PS3 designated as the type strain. Our results advance the understanding of beneficial plant-microbe interactions in cereals and highlight the promise of native Pseudomonas species as biocontrol agents for sustainable disease management.