# Therapeatic evaluation and single cell analysis of adipose stromal vascular fraction isolation from a commercial cell separation system

## TL;DR

This study evaluates a commercial system for isolating adipose-derived cells and finds it effective for wound healing due to specific cell types.

## Contribution

The study introduces a commercial method for isolating SVF cells with therapeutic potential for wound repair.

## Key findings

- C-SVF showed comparable cell yield to L-SVF and better in vitro proliferation and reduced apoptosis.
- C-SVF improved wound closure, collagen remodeling, and angiogenesis in a mouse model.
- snRNA-seq revealed enrichment of APOE+ ADSCs and M2 macrophages in C-SVF, promoting wound repair.

## Abstract

In the field of regenerative therapy, the stromal vascular fraction (SVF) extracted from adipose tissue has been widely recognized for its significant benefits. However, the cellular composition and therapeutic effect of SVF products prepared via different methods are unclear.

SVF cells were obtained via three approaches: (1) generation of the SVF via mechanical emulsification (M-SVF), (2) generation of the SVF via laboratory enzymatic digestion (L-SVF), and (3) generation of the SVF via commercial cell separation systems (C-SVF). We evaluated their healing effects on mouse wounds. Additionally, we utilized single-nucleus RNA sequencing (snRNA-seq) technology to explore the cellular composition of the C-SVF.

The cell yield of C-SVF was comparable to that of L-SVF. During in vitro culture, C-SVF exhibited enhanced proliferation and a reduced proportion of apoptotic cells. In a mouse wound model, the application of C-SVF facilitated the closure of mouse wounds and improved collagen remodeling and angiogenesis in the wound area. Additional snRNA-seq analysis revealed that APOE + adipose-derived stem cells and immune cells, especially M2 anti-inflammatory macrophages, are enriched in C-SVF, which together promote wound repair, and that APOE + adipose-derived stem cells (ADSCs) and immune cells, especially M2 anti-inflammatory macrophages, are enriched in C-SVF, which jointly regulate and promote wound repair.

A commercial extraction system is an effective method for isolating viable SVF cells enriched with APOE + ADSCs and M2 macrophages.