# Genomic, Proteomic, and Biochemical Study of Pleurotus pulmonarius Secretome and Its Role in Biomass Saccharification

## TL;DR

This study explores how the mushroom Pleurotus pulmonarius breaks down plant material using enzymes, showing its potential for sustainable biomass conversion.

## Contribution

The study reveals the secretome dynamics of P. pulmonarius on different substrates and demonstrates its effectiveness in biomass saccharification.

## Key findings

- Beechwood cultivation induced the richest secretome with abundant oxidases.
- Corn stover secretome achieved higher lignocellulose saccharification despite lower enzyme diversity.
- Adding P. pulmonarius corn stover secretomes to industrial enzymes increased sugar release by 40%.

## Abstract

Pleurotus species include edible mushrooms that
grow on a range of lignocellulosic substrates by secreting hydrolytic
and oxidative enzymes. This flexibility offers opportunities to valorize
agro-industrial residues for sustainable production of food and biomaterials.
In this study, we analyzed the genome of Pleurotus
pulmonarius and its biocatalytic potential for lignocellulose
saccharification using proteomics and biochemical assays. The fungus
was cultivated on beechwood, corn stover, and xylose. Beechwood induced
the richest secretome with abundant oxidases. The corn stover secretome
had fewer proteins but was focused on carbohydrate-acting enzymes,
with abundant polysaccharide-degrading and accessory enzymes. Despite
lower enzyme diversity, corn stover secretome achieved higher lignocellulose
saccharification, further improved by oxidoreductase inhibition. Supplementing
an industrial cellulase cocktail with P. pulmonarius corn stover secretomes enhanced sugar release by 40%. These findings
highlight the dynamic enzymatic response of P. pulmonarius to lignocellulosic substrates and its potential in biomass valorization
and the design of enzymatic cocktails.