# The locking mechanism of human TRPV6 inhibition by intracellular magnesium

## TL;DR

The study reveals how intracellular magnesium ions inhibit the TRPV6 calcium channel, potentially aiding in treating diseases linked to its overactivity.

## Contribution

The novel contribution is the identification of specific magnesium binding sites and their role in locking TRPV6 in a closed state.

## Key findings

- Intracellular Mg2+ binds to four sites around the TRPV6 pore entrance.
- Mg2+ binding prevents structural changes in S6, maintaining the channel in a closed state.
- This mechanism could inform strategies for treating TRPV6-associated diseases.

## Abstract

TRPV6 is a member of the vanilloid subfamily of transient receptor potential channels, which serves as the master regulator of Ca2+ homeostasis. TRPV6 functions as a constitutively active Ca2+ channel, and emerging evidence indicates that its overactivity underpins the progression of several human diseases, including cancer. Hence, there is a pressing need to identify TRPV6 inhibitors in conjunction with a deep mechanistic understanding of their effects on the channel activity. Here we combine cryo-electron microscopy, mutagenesis, electrophysiology and molecular dynamics modeling to decipher the molecular mechanism of TRPV6 inhibition by intracellular Mg2+. Mg2+ appears to bind to four, one per subunit, sites around the intracellular entrance to the TRPV6 channel pore, contributed by the negatively charged residues, D489 in the transmembrane helix S5 and D580 in S6. When bound to the D489-D580 site, Mg2+ prevents the α-to-π transition in the middle of S6 that accompanies channel opening, thus maintaining S6 entirely α-helical, locking the channel in the closed state and inhibiting TRPV6-mediated currents. Further exploration of this inhibitory mechanism may help to develop future strategies for the treatment of TRPV6-associated diseases.

TRP channel TRPV6 is a regulator of Ca2+ homeostasis. Here, authors decipher the mechanism of TRPV6 inhibition by intracellular Mg2+ ions that bind to four sites around the intracellular pore entrance and lock the channel in the closed state.