# Two-step purification of elastin-like polypeptide-fusion superoxide dismutase via hydrophobicity and thermoresponsiveness

## TL;DR

This paper introduces a two-step purification method for an antioxidant enzyme using ELP tags, offering a faster and cheaper alternative to traditional methods.

## Contribution

A novel two-step purification strategy using ELP hydrophobicity and thermoresponsiveness for SOD fusion proteins is developed.

## Key findings

- Foam separation achieved 85.67% protein recovery and 93.32% activity recovery.
- Inverse transition cycling yielded a 91.98% recovery rate and 17.45 purification fold.
- The combined method achieved 85.84% total yield and 37.52 overall purification fold.

## Abstract

Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to oxygen and hydrogen peroxide, serving as a key antioxidant enzyme with important therapeutic and industrial applications. However, the purification of recombinant SOD remains challenging due to low expression levels and the complexity of traditional purification methods, which involve time-consuming and multi-step chromatography. Elastin-like polypeptides (ELPs) offer a promising alternative due to their hydrophobic and thermoresponsive properties, which can be leveraged for non-chromatographic purification.

A fusion protein of human SOD and an ELP tag (hSOD-ELP) was produced. The purification strategy consisted of two sequential steps. First, foam separation was employed, exploiting the hydrophobicity of the ELP to selectively adsorb hSOD-ELP at the gas-liquid interface. Second, inverse transition cycling (ITC) was used to further purify hSOD-ELP by exploiting ELP’s thermoresponsiveness.

Under optimized conditions (0.4 mg/mL protein, 30 °C), the initial foam separation step achieved an enrichment ratio of 1.93, a protein recovery of 85.67%, an enzyme activity enrichment of 2.15, and an activity recovery of 93.32%. The subsequent ITC step yielded a recovery rate of 91.98% and a purification fold of 17.45. The cumulative two-step process resulted in a total yield of 85.84% and overall purification fold of 37.52, yielding the purified hSOD-ELP with a final purity of approximately 85%.

These results demonstrate that ELP-mediated purification offers a scalable and economical alternative to conventional methods. The combination of foam separation and thermal precipitation minimizes the need for expensive chromatography, making this strategy particularly promising for industrial-scale biotechnological applications.