# Scorpion-Centipede extracts mitigate ovariectomy-induced osteoporosis in mice through facilitating Cx3cr1 expression

## TL;DR

A traditional Chinese medicine formula containing Scorpion and Centipede helps treat postmenopausal osteoporosis in mice by boosting bone formation and reducing fat formation through a specific gene.

## Contribution

The study reveals that Scorpion-Centipede promotes bone mass via Cx3cr1, offering a novel mechanism for treating postmenopausal osteoporosis.

## Key findings

- SC treatment improved bone mineral density and other bone parameters in ovariectomized mice.
- SC increased osteogenic genes and decreased adipogenic genes in bone tissue and BMMSCs.
- Cx3cr1 knockdown blocked the osteogenic and adipogenic effects of SC in cell lines.

## Abstract

Scorpion and Centipede (SC) is an ancient formula of traditional Chinese medicine that is commonly utilized in a range of disorders, and it has been shown to have pharmacological effects on postmenopausal osteoporosis (PMOP). However, the specific mechanism of SC for the treatment of PMOP remains to be further investigated. This study aimed to investigate the therapeutic potential of a traditional Chinese medicine formula consisting of SC in regulating the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) to treat PMOP.

The ovariectomy-induced mice (OVX) were established and divided into a sham surgery group, OVX, OVX with alendronate sodium, and OVX with SC, and kept for 10 weeks. In vitro experiments were conducted to evaluate the effects of SC on osteogenic and adipogenic differentiation in BMMSCs, MC3T3-E1, and 3T3-L1 cells.

The results showed that SC treatment significantly improved bone mineral density (BMD), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), trabecular number (Tb.N), bone volume fraction (BV/TV), and trabecular pattern factor (Tb.Pf) in OVX mice. In addition, SC treatment markedly increased Runx2, Osx, Alp, and Cx3cr1 while decreasing adipogenic genes like PPARγ and C/EBPα in the bone tissue of OVX mice and BMMSCs. Notably, the effects of SC on osteogenic and adipogenic genes were blocked in Cx3cr1 knockdown MC3T3-E1 and 3T3-L1 cells.

This study demonstrates that the SC effectively increases bone mass and osteogenesis by promoting Cx3cr1, thereby increasing osteogenic differentiation and inhibiting adipogenic differentiation. These findings amplified the mechanisms of SC and its potential to treat PMOP.

Diagram showing the impact of Scorpion-Centipede on OVX mice and BMMSCs. It illustrates the role of Cx3cr1 in enhancing osteogenic effects and reducing adipogenic effects. Arrows indicate the process flow between elements.